Abstract: BACKGROUND: Dermatophytes are filamentous keratinophilic fungi. Trichophyton rubrum is a prevalent infectious agent in tineas and other skin diseases. Drug therapy is considered to be limited in the treatment of such infections, mainly due to low accessibility of the drug to the tissue attacked and development of antifungal resistance in these microorganisms. In this context, Photodynamic Therapy is presented as an alternative. OBJECTIVE: Evaluate, in vitro, the photodynamic activity of four derivatives of Protoporphyrin IX by irradiation with LED 400 nm in T. rubrum. METHOD: Assays were subjected to irradiation by twelve cycles of ten minutes at five minute intervals. RESULT: Photodynamic action appeared as effective with total elimination of UFCs from the second irradiation cycle. CONCLUSION: Studies show that the photodynamic activity on Trichophyton rubrum relates to a suitable embodiment of the photosensitizer, which can be maximized by functionalization of peripheral groups of the porphyrinic ring.

INTRODUCTION: Ozone is a potent antibacterial agent. Because ozone oxidizes organic material, it directly attacks microorganisms resulting in safe, fast and economical sterilization at low temperatures. This study evaluated the efficacy of ozonated water in the reprocessing of dialyzers obtained from a hemodialysis service in São José do Rio Preto. METHODS: A total of 23 blood dialyzers were collected at the conclusion of the morning hemodialysis shift. The equipment was collected after the maximum number of reuses, with the last disinfection performed with purified water (obtained by reverse osmosis and subsequent reverse ultrafiltration). The number and species of microorganisms in the dialyzers were evaluated before and after treatment with ozonated water. The ozonation of sterile deionized water was achieved by direct contact between the water and the ozone generating equipment. RESULTS:The mean number of microorganisms before sterilization was 1,47.10(9) colony forming units per ml (CFU/ml). After treatment with ozonated water, the number of microorganisms was 4,80.10¹ CFU/ml. CONCLUSION: Ozonated water is an effective decontaminant for most blood dialyzers.

INTRODUÇÃO: Microsporum canis é o causador mais comum de dermatofitose canina e felina apresentando assim um importante papel zoonótico. OBJETIVOS: o presente estudo teve como objetivo determinar a ação antifúngica de extratos de plantas medicinais e óleo de eucalipto frente ao fungo patogênico Microsporum canis. MÉTODOS: os extratos brutos foram preparados utilizando-se 300 g de folhas, previamente lavadas e 450 mL de água deionizada. O material vegetal foi misturado, triturado, coado e filtrado e em seguida, esterilizados e conservados a 10 ± 2 ºC. Depois de esterilizado, o meio Sabouraud dextrose (Difco) atingiu a temperatura de 55 ± 1 ºC. Foram adicionados 15 mL em placas de Petri contendo os extratos em concentrações que correspondiam a um, dois, três, quatro e cinco ml. Uma vez solidificado o meio, o fungo foi inoculado. As placas inoculadas foram mantidas em incubadora B.O.D. a temperatura de 36 ± 0,5 ºC até o desenvolvimento do fungo na placa de controle. RESULTADOS: os extratos de romã, manga e eucalipto diminuíram o crescimento do fungo, mas os de citronela, cravo de defunto, arruda, tiririca, graviola e folhas e flores de calêndula, promoveram o desenvolvimento do fungo. O restante dos extratos e o óleo de eucalipto, não apresentaram ação fungicida nem promoveram o crescimento micelial. CONCLUSÕES: a maioria dos extratos testados e o óleo de eucalipto não apresentam efeito inibitório no desenvolvimento de Microsporum canis tornando inutilizável o uso destes fitoterápicos no controle deste fungo; enquanto que os extratos de citronela, cravo de defunto, arruda, tiririca, graviola e de folhas e flores de calêndula induziram o desenvolvimento do fungo, mostrando que a utilização de fitoterápicos deve ser realizada de uma maneira correta pois em caso contrário podese agravar o problema. Os extratos de manga, romã e eucalipto podem ser usados como substâncias fungistáticas no controle de M. canis.

INTRODUCTION: Microsporum canis is the most common cause of canine and feline dermatophytosis and thus has an important zoonotic role. OBJECTIVES: the aim of this study was to determine the antifungal action of medicinal plant extracts and of eucalyptus oil against pathogenic fungus Microsporum canis. METHODS: the extracts were prepared by mixing 300 g of previously washed leaves with 450 mL of distilled water. Then the material was triturated, filtered, sterilized and conserved at 10 + 2 ºC. Fifteen milliliters of sterilized medium Sabouraud dextrose (Difco) at a temperature of 55 + 1 ºC was added in Petri dishes containing the extracts in one, two, three, four and five mm concentrations. The fungus was inoculated once the medium was solidified. The inoculated dishes were maintained in B.O.D. incubator at 36 ± 0,5 ºC until the fungus developed in the controls. RESULTS: the extracts from Punica granatum, Mangifera indica and Eucalyptus spp reduced the growth of fungus, but the extracts from Cymgopogom nardus, Tagetes minuta, Ruta graviolens, Cyperus rotundus, Annona moricata and Calendula spp leaves and flowers boosted the growth of fungus. The other extracts and the eucalyptus oil neither show any fungicidal action nor encourage mycelium growth. CONCLUSIONS: the use of most tested extracts and eucalyptus oil is not suitable for the treatment of Microsporum canis dermatophytosis due to lack of inhibitory effects. The extracts from Cymgopogom nardus, Tagetes minuta, Ruta graviolens, Cyperus rotundus, Annona moricata and from of Calendula spp leaves and flowers help the development of the fungus making clear that phytotherapy should be properly used, otherwise it can worsen the problem. However; extracts from Mangifera indica, Punica granatum and Eucalyptus spp. can be used as fungistatic.